Survey Autoradiography Using Polaroid Land Films

Low-cost conversion of the Polaroid MD-4 land camera to a digital gel documentation system.

A simple, inexpensive design is presented for the rapid conversion of the popular MD-4 Polaroid land camera to a high quality digital gel documentation system. Images of ethidium bromide stained DNA gels captured using the digital system were compared to images captured on Polaroid instant film. Resolution and sensitivity were enhanced using the digital system. In addition to the low cost and s...

Simulate the Polaroid transfer effect, digitally

Most readers will probably be familiar with Polaroid instant picture products – you push the button and the print is ejected and develops right before your eyes. For many years professional image-makers have been using the unique features of this technology to create wonderfully textured images. The process involved substituting watercolor paper for the printing surface supplied by Polaroid. As...

Quantitative autoradiography using a personal computer.

A video digitizing system based on an Apple II personal computer has been developed for use in quantitative autoradiography. The selection process of an acceptable video camera, an illumination system, and the functions provided in the software are discussed. This system is in routine use and has been duplicated by several other laboratories.

Polaroid print film for fluorescein angiography.

Class I HDAC imaging using [ (3)H]CI-994 autoradiography.

[ (3)H]CI-994, a radioactive isotopologue of the benzamide CI-994, a class I histone deacetylase inhibitor (HDACi), was evaluated as an autoradiography probe for ex vivo labeling and localizing of class I HDAC (isoforms 1-3) in the rodent brain. After protocol optimization, up to 80% of total binding was attributed to specific binding. Notably, like other benzamide HDACi, [ (3)H]CI-994 exhibits...